R-Spondin 3 ELISA Kit

Produktnummer ABIN2044373

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human R-Spondin 3.

Kurzübersicht für R-Spondin 3 ELISA Kit (ABIN2044373)

Target: R-Spondin 3 (RSPO3)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails R-Spondin 3 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.128 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: R-Spondin 3 (RSPO3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 3 (RSPO3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 3 (RSPO3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 3 (RSPO3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of R-Spondin 3 (RSPO3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu R-Spondin 3

Target: R-Spondin 3 (RSPO3)