PC ELISA Kit

Produktnummer ABIN2044367

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human PC.

Kurzübersicht für PC ELISA Kit (ABIN2044367)

Target: PC (Pyruvate Carboxylase (PC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails PC ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.63 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Pyruvate Carboxylase (PC) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Carboxylase (PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Carboxylase (PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Carboxylase (PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pyruvate Carboxylase (PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PC

Target: PC (Pyruvate Carboxylase (PC))