MPZ ELISA Kit

Produktnummer ABIN2044357

Ratte MPZ ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte MPZ.

Kurzübersicht für MPZ ELISA Kit (ABIN2044357)

Target: MPZ (Myelin Protein Zero (MPZ))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails MPZ ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.122 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Protein Zero, Myelin (MPZ) BioAssay-ELISA Kit (Rat) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Zero, Myelin (MPZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Zero, Myelin (MPZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MPZ

Target: MPZ (Myelin Protein Zero (MPZ))

Andere Bezeichnung: Protein Zero, Myelin (MPZ)