PCMT1 ELISA Kit

Produktnummer ABIN2044347

Das Human PCMT1 ELISA Kit (ABIN2044347) ist ein Colorimetric ELISA Kit zur Detektion von Human PCMT1.

Kurzübersicht für PCMT1 ELISA Kit (ABIN2044347)

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 pg/mL

Applikation: ELISA

Produktdetails PCMT1 ELISA Kit

Untere Nachweisgrenze: 31.25 pg/mL

Sensitivität: < 13.8 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Protein L-Isoaspartate-O-Methyltransferase (PCMT1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein L-Isoaspartate-O-Methyltransferase (PCMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein L-Isoaspartate-O-Methyltransferase (PCMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein L-Isoaspartate-O-Methyltransferase (PCMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein L-Isoaspartate-O-Methyltransferase (PCMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PCMT1

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Andere Bezeichnung: Protein L-Isoaspartate-O-Methyltransferase (PCMT1)

Pathways: Ribonucleoprotein Complex Subunit Organization