PRKX ELISA Kit

Produktnummer ABIN2044345

Ratte PRKX ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte PRKX.

Kurzübersicht für PRKX ELISA Kit (ABIN2044345)

Target: PRKX (Protein Kinase, X-Linked (PRKX))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails PRKX ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Marke: BioAssay™

Sensitivität: < 0.60 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Protein Kinase, X-Linked (PRKX) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, X-Linked (PRKX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, X-Linked (PRKX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, X-Linked (PRKX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protein Kinase, X-Linked (PRKX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PRKX

Target: PRKX (Protein Kinase, X-Linked (PRKX))

Pathways: Thyroid Hormone Synthesis