PSME3 ELISA Kit

Produktnummer ABIN2044317

Das Human PSME3 ELISA Kit (ABIN2044317) ist ein Colorimetric ELISA Kit zur Detektion von Human PSME3.

Kurzübersicht für PSME3 ELISA Kit (ABIN2044317)

Target: PSME3

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails PSME3 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.054 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Proteasome Activator Subunit 3 (PSME3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Activator Subunit 3 (PSME3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Activator Subunit 3 (PSME3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Activator Subunit 3 (PSME3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Proteasome Activator Subunit 3 (PSME3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PSME3

Target: PSME3

Andere Bezeichnung: Proteasome Activator Subunit 3 (PSME3)

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Hepatitis C, Synthesis of DNA