F2RL3 ELISA Kit

Produktnummer ABIN2044313

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Ratte F2RL3.

Kurzübersicht für F2RL3 ELISA Kit (ABIN2044313)

Target: F2RL3 (Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails F2RL3 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Protease Activated Receptor 4 (PAR4) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 4 (PAR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 4 (PAR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 4 (PAR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Protease Activated Receptor 4 (PAR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu F2RL3

Target: F2RL3 (Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3))

Andere Bezeichnung: Protease Activated Receptor 4 (PAR4)

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process