Prostacyclin Receptor ELISA Kit

Produktnummer ABIN2044301

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Prostacyclin Receptor.

Kurzübersicht für Prostacyclin Receptor ELISA Kit (ABIN2044301)

Target: Prostacyclin Receptor (PTGIR)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails Prostacyclin Receptor ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.071 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Prostacyclin Receptor (PTGIR) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostacyclin Receptor (PTGIR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostacyclin Receptor (PTGIR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostacyclin Receptor (PTGIR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Prostacyclin Receptor (PTGIR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Prostacyclin Receptor

Target: Prostacyclin Receptor (PTGIR)

Pathways: cAMP Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling