PIP ELISA Kit

Produktnummer ABIN2044285

Ratte PIP ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte PIP.

Kurzübersicht für PIP ELISA Kit (ABIN2044285)

Target: PIP (Prolactin-Induced Protein (PIP))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 46.87-3000 pg/mL

Applikation: ELISA

Produktdetails PIP ELISA Kit

Untere Nachweisgrenze: 46.87 pg/mL

Sensitivität: < 16.23 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Prolactin Induced Protein (PIP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin Induced Protein (PIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolactin Induced Protein (PIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolactin Induced Protein (PIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Prolactin Induced Protein (PIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PIP

Target: PIP (Prolactin-Induced Protein (PIP))

Andere Bezeichnung: Prolactin Induced Protein (PIP)