PIBF1 ELISA Kit

Produktnummer ABIN2044275

Das Human PIBF1 ELISA Kit (ABIN2044275) ist ein Colorimetric ELISA Kit zur Detektion von Human PIBF1.

Kurzübersicht für PIBF1 ELISA Kit (ABIN2044275)

Target: PIBF1 (Progesterone Immunomodulatory Binding Factor 1 (PIBF1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails PIBF1 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.116 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Progesterone Immunomodulatory Binding Factor 1 (PIBF1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Progesterone Immunomodulatory Binding Factor 1 (PIBF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Progesterone Immunomodulatory Binding Factor 1 (PIBF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Progesterone Immunomodulatory Binding Factor 1 (PIBF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Progesterone Immunomodulatory Binding Factor 1 (PIBF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PIBF1

Target: PIBF1 (Progesterone Immunomodulatory Binding Factor 1 (PIBF1))