KCNJ5 ELISA Kit

Produktnummer ABIN2044257

Dieses Ratte KCNJ5 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte KCNJ5 zu quantifizieren.

Kurzübersicht für KCNJ5 ELISA Kit (ABIN2044257)

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails KCNJ5 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.055 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu KCNJ5

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Andere Bezeichnung: Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5)

Pathways: Notch Signalweg