WBP2NL ELISA Kit

Produktnummer ABIN2044253

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Rind (Kuh) WBP2NL.

Kurzübersicht für WBP2NL ELISA Kit (ABIN2044253)

Target: WBP2NL (WBP2 N-terminal Like (WBP2NL))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails WBP2NL ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Postacrosomal Sheath WW Domain Binding Protein (PAWP) BioAssay-ELISA Kit (Bovine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Postacrosomal Sheath WW Domain Binding Protein (PAWP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Postacrosomal Sheath WW Domain Binding Protein (PAWP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Postacrosomal Sheath WW Domain Binding Protein (PAWP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Postacrosomal Sheath WW Domain Binding Protein (PAWP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu WBP2NL

Target: WBP2NL (WBP2 N-terminal Like (WBP2NL))

Andere Bezeichnung: Postacrosomal Sheath WW Domain Binding Protein (PAWP)