PABPC1L ELISA Kit

Produktnummer ABIN2044249

Human PABPC1L ELISA Kit, Colorimetric Assay für die Quantifizierung von Human PABPC1L.

Kurzübersicht für PABPC1L ELISA Kit (ABIN2044249)

Target: PABPC1L (Poly(A) Binding Protein, Cytoplasmic 1-Like (PABPC1L))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails PABPC1L ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.115 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PABPC1L

Target: PABPC1L (Poly(A) Binding Protein, Cytoplasmic 1-Like (PABPC1L))

Andere Bezeichnung: Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L)