Podoplanin ELISA Kit

Produktnummer ABIN2044247

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus Podoplanin.

Kurzübersicht für Podoplanin ELISA Kit (ABIN2044247)

Target: Podoplanin (PDPN)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails Podoplanin ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.055 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Podoplanin (PDPN) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podoplanin (PDPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Podoplanin (PDPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Podoplanin (PDPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Podoplanin (PDPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Podoplanin

Target: Podoplanin (PDPN)

Pathways: Dicarboxylic Acid Transport