PODXL ELISA Kit

Produktnummer ABIN2044245

Dieses Ratte PODXL ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte PODXL zu quantifizieren.

Kurzübersicht für PODXL ELISA Kit (ABIN2044245)

Target: PODXL (Podocalyxin-Like (PODXL))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails PODXL ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.61 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Podocalyxin (PCX) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podocalyxin (PCX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Podocalyxin (PCX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Podocalyxin (PCX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Podocalyxin (PCX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Andere Bezeichnung: Podocalyxin (PCX)

Pathways: Tube Formation