PLA2G12B ELISA Kit

Produktnummer ABIN2044223

Produktdetails PLA2G12B ELISA Kit

Target: PLA2G12B (Phospholipase A2, Group XIIB (PLA2G12B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.058 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Phospholipase A2, Group XIIB (PLA2G12B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group XIIB (PLA2G12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group XIIB (PLA2G12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phospholipase A2, Group XIIB (PLA2G12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PLA2G12B

Target: PLA2G12B (Phospholipase A2, Group XIIB (PLA2G12B))

Abstract: PLA2G12B Produkte

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