PLA2G3 ELISA Kit

Produktnummer ABIN2044217

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus PLA2G3.

Kurzübersicht für PLA2G3 ELISA Kit (ABIN2044217)

Target: PLA2G3 (Phospholipase A2, Group III (PLA2G3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails PLA2G3 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.27 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Phospholipase A2, Group III (PLA2G3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group III (PLA2G3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group III (PLA2G3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group III (PLA2G3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phospholipase A2, Group III (PLA2G3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PLA2G3

Target: PLA2G3 (Phospholipase A2, Group III (PLA2G3))