PDE1A ELISA Kit

Produktnummer ABIN2044213

Human PDE1A ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human PDE1A.

Kurzübersicht für PDE1A ELISA Kit (ABIN2044213)

Target: PDE1A (Phosphodiesterase 1A (PDE1A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails PDE1A ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.126 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 1A, Calmodulin Dependent (PDE1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Phosphodiesterase 1A, Calmodulin Dependent (PDE1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PDE1A

Target: PDE1A (Phosphodiesterase 1A (PDE1A))

Andere Bezeichnung: Phosphodiesterase 1A, Calmodulin Dependent (PDE1A)

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma