PER2 ELISA Kit

Produktnummer ABIN2044197

Dieses Ratte PER2 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte PER2 zu quantifizieren.

Kurzübersicht für PER2 ELISA Kit (ABIN2044197)

Target: PER2 (Period Circadian Protein 2 (PER2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails PER2 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.101 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Period Circadian Protein 2 (PER2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Period Circadian Protein 2 (PER2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Period Circadian Protein 2 (PER2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Period Circadian Protein 2 (PER2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Period Circadian Protein 2 (PER2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PER2

Target: PER2 (Period Circadian Protein 2 (PER2))