PGC ELISA Kit
Kurzübersicht für PGC ELISA Kit (ABIN2044183)
Target
Alle PGC ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
-
-
Untere Nachweisgrenze
- 0.15 ng/mL
-
Sensitivität
- < 0.063 ng/mL
-
-
-
-
Plattentyp
- Pre-coated
-
Protokoll
- Pepsinogen C (PGC) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsinogen C (PGC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pepsinogen C (PGC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pepsinogen C (PGC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pepsinogen C (PGC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Lagerung
- 4 °C/-20 °C
-
-
- PGC (Pepsinogen C (PGC))
Target Alle PGC ELISA Kits anzeigen
-