PDLIM1 ELISA Kit

Produktnummer ABIN2044181

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human PDLIM1.

Kurzübersicht für PDLIM1 ELISA Kit (ABIN2044181)

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails PDLIM1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.061 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: PDZ And LIM Domain Protein 1 (PDLIM1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDZ And LIM Domain Protein 1 (PDLIM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PDZ And LIM Domain Protein 1 (PDLIM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDZ And LIM Domain Protein 1 (PDLIM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of PDZ And LIM Domain Protein 1 (PDLIM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PDLIM1

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Andere Bezeichnung: PDZ And LIM Domain Protein 1 (PDLIM1)