KIAA0101 ELISA Kit

Produktnummer ABIN2044179

Dieses Human KIAA0101 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human KIAA0101 zu quantifizieren.

Kurzübersicht für KIAA0101 ELISA Kit (ABIN2044179)

Target: KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails KIAA0101 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.27 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: PCNA Associated Factor 15kDa (PAF) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCNA Associated Factor 15kDa (PAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PCNA Associated Factor 15kDa (PAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCNA Associated Factor 15kDa (PAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of PCNA Associated Factor 15kDa (PAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu KIAA0101

Target: KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))

Andere Bezeichnung: PCNA Associated Factor 15kDa (PAF)

Pathways: Chromatin Binding