PNPLA3 ELISA Kit

Produktnummer ABIN2044177

Ratte PNPLA3 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte PNPLA3.

Kurzübersicht für PNPLA3 ELISA Kit (ABIN2044177)

Target: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails PNPLA3 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.057 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu PNPLA3

Target: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Andere Bezeichnung: Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3)