SP7 ELISA Kit

Produktnummer ABIN2044149

Dieses Maus SP7 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus SP7 zu quantifizieren.

Kurzübersicht für SP7 ELISA Kit (ABIN2044149)

Target: SP7 (Sp7 Transcription Factor (SP7))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails SP7 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.064 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Osterix (OSX) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osterix (OSX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osterix (OSX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osterix (OSX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Osterix (OSX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SP7

Target: SP7 (Sp7 Transcription Factor (SP7))

Andere Bezeichnung: Osterix (OSX)