OGN ELISA Kit

Produktnummer ABIN2044145

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus OGN.

Kurzübersicht für OGN ELISA Kit (ABIN2044145)

Target: OGN (Osteoglycin (OGN))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails OGN ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.066 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Osteoglycin (OGN) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteoglycin (OGN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteoglycin (OGN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteoglycin (OGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Osteoglycin (OGN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu OGN

Target: OGN (Osteoglycin (OGN))

Pathways: Glycosaminoglycan Metabolic Process