OAT ELISA Kit

Produktnummer ABIN2044137

Dieses Human OAT ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human OAT zu quantifizieren.

Kurzübersicht für OAT ELISA Kit (ABIN2044137)

Target: OAT (Ornithine Aminotransferase (OAT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails OAT ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.059 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ornithine Aminotransferase (OAT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Aminotransferase (OAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Aminotransferase (OAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Aminotransferase (OAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ornithine Aminotransferase (OAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu OAT

Target: OAT (Ornithine Aminotransferase (OAT))