NCOA3 ELISA Kit

Produktnummer ABIN2044127

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte NCOA3.

Kurzübersicht für NCOA3 ELISA Kit (ABIN2044127)

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails NCOA3 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.115 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Nuclear Receptor Coactivator 3 (NCOA3) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Coactivator 3 (NCOA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nuclear Receptor Coactivator 3 (NCOA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha