NOTCH2 ELISA Kit

Produktnummer ABIN2044111

Human NOTCH2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human NOTCH2.

Kurzübersicht für NOTCH2 ELISA Kit (ABIN2044111)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Applikation: ELISA

Produktdetails NOTCH2 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Sensitivität: < 0.33 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Notch Homolog 2 (NOTCH2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Notch Homolog 2 (NOTCH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Notch Homolog 2 (NOTCH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Notch Homolog 2 (NOTCH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Notch Homolog 2 (NOTCH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Andere Bezeichnung: Notch Homolog 2 (NOTCH2)

Pathways: Notch Signalweg, Stem Cell Maintenance