NONO ELISA Kit

Produktnummer ABIN2044103

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human NONO.

Kurzübersicht für NONO ELISA Kit (ABIN2044103)

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails NONO ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.126 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Non POU Domain Containing Octamer Binding Protein (NONO) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Non POU Domain Containing Octamer Binding Protein (NONO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Non POU Domain Containing Octamer Binding Protein (NONO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Andere Bezeichnung: Non POU Domain Containing Octamer Binding Protein (NONO)