Neutrophil Alkaline Phosphatase ELISA Kit

Produktnummer ABIN2044089

Dieses Human Neutrophil Alkaline Phosphatase ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human Neutrophil Alkaline Phosphatase zu quantifizieren.

Kurzübersicht für Neutrophil Alkaline Phosphatase ELISA Kit (ABIN2044089)

Target: Neutrophil Alkaline Phosphatase (NAP)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails Neutrophil Alkaline Phosphatase ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.57 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Neutrophil Alkaline Phosphatase (NAP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Alkaline Phosphatase (NAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Alkaline Phosphatase (NAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Alkaline Phosphatase (NAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neutrophil Alkaline Phosphatase (NAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Neutrophil Alkaline Phosphatase

Target: Neutrophil Alkaline Phosphatase (NAP)