NEFH ELISA Kit

Produktnummer ABIN2044075

Human NEFH ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human NEFH.

Kurzübersicht für NEFH ELISA Kit (ABIN2044075)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Applikation: ELISA

Produktdetails NEFH ELISA Kit

Untere Nachweisgrenze: 15.62 pg/mL

Sensitivität: < 5.2 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Neurofilament, Heavy Polypeptide (NEFH) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament, Heavy Polypeptide (NEFH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Neurofilament, Heavy Polypeptide (NEFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))