NQO1 ELISA Kit

Produktnummer ABIN2044045

Maus NQO1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus NQO1.

Kurzübersicht für NQO1 ELISA Kit (ABIN2044045)

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails NQO1 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.127 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: NADH Dehydrogenase, Quinone 1 (NQO1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase, Quinone 1 (NQO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADH Dehydrogenase, Quinone 1 (NQO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase, Quinone 1 (NQO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of NADH Dehydrogenase, Quinone 1 (NQO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NQO1

Target: NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

Andere Bezeichnung: NADH Dehydrogenase, Quinone 1 (NQO1)