NAT8L ELISA Kit

Produktnummer ABIN2044027

Human NAT8L ELISA Kit, Colorimetric Assay für die Quantifizierung von Human NAT8L.

Kurzübersicht für NAT8L ELISA Kit (ABIN2044027)

Target: NAT8L (N-Acetyltransferase 8-Like (GCN5-Related, Putative) (NAT8L))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails NAT8L ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.064 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: N-Acetyltransferase 8 Like Protein (NAT8L) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acetyltransferase 8 Like Protein (NAT8L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acetyltransferase 8 Like Protein (NAT8L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acetyltransferase 8 Like Protein (NAT8L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of N-Acetyltransferase 8 Like Protein (NAT8L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NAT8L

Target: NAT8L (N-Acetyltransferase 8-Like (GCN5-Related, Putative) (NAT8L))

Andere Bezeichnung: N-Acetyltransferase 8 Like Protein (NAT8L)