MYL3/CMLC1 ELISA Kit

Produktnummer ABIN2044013

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human MYL3/CMLC1.

Kurzübersicht für MYL3/CMLC1 ELISA Kit (ABIN2044013)

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78-5000 pg/mL

Applikation: ELISA

Produktdetails MYL3/CMLC1 ELISA Kit

Untere Nachweisgrenze: 78 pg/mL

Analytische Methode: Quantitative

Sensitivität: < 28 pg/mL

Bestandteile: A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: The Human Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MYL3 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYL3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MYL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MYL3 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MYL3/CMLC1

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Andere Bezeichnung: Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3)