CCDC177 ELISA Kit

Produktnummer ABIN2044003

Human CCDC177 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human CCDC177.

Kurzübersicht für CCDC177 ELISA Kit (ABIN2044003)

Target: CCDC177 (Coiled-Coil Domain Containing 177 (CCDC177))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails CCDC177 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.112 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Myelin Proteolipid Protein Like Protein (PLPL) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Proteolipid Protein Like Protein (PLPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myelin Proteolipid Protein Like Protein (PLPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myelin Proteolipid Protein Like Protein (PLPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Myelin Proteolipid Protein Like Protein (PLPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CCDC177

Target: CCDC177 (Coiled-Coil Domain Containing 177 (CCDC177))

Andere Bezeichnung: Myelin Proteolipid Protein Like Protein (PLPL)