MUC12 ELISA Kit

Produktnummer ABIN2043991

Human MUC12 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human MUC12.

Kurzübersicht für MUC12 ELISA Kit (ABIN2043991)

Target: MUC12 (Mucin 12, Cell Surface Associated (MUC12))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails MUC12 ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.132 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Mucin 12, Cell Surface Associated (MUC12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 12, Cell Surface Associated (MUC12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 12, Cell Surface Associated (MUC12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 12, Cell Surface Associated (MUC12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mucin 12, Cell Surface Associated (MUC12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MUC12

Target: MUC12 (Mucin 12, Cell Surface Associated (MUC12))