Monoamine Oxidase A ELISA Kit

Produktnummer ABIN2043987

Dieses Ratte Monoamine Oxidase A ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte Monoamine Oxidase A zu quantifizieren.

Kurzübersicht für Monoamine Oxidase A ELISA Kit (ABIN2043987)

Target: Monoamine Oxidase A (MAOA)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125-8000 pg/mL

Applikation: ELISA

Produktdetails Monoamine Oxidase A ELISA Kit

Untere Nachweisgrenze: 125 pg/mL

Sensitivität: < 55 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Monoamine Oxidase A (MAOA) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monoamine Oxidase A (MAOA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monoamine Oxidase A (MAOA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monoamine Oxidase A (MAOA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Monoamine Oxidase A (MAOA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Monoamine Oxidase A

Target: Monoamine Oxidase A (MAOA)