MFGE8 ELISA Kit

Produktnummer ABIN2043977

Produktdetails MFGE8 ELISA Kit

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 6.7 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Milk Fat Globule EGF Factor 8 (MFGE8) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Milk Fat Globule EGF Factor 8 (MFGE8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Milk Fat Globule EGF Factor 8 (MFGE8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Andere Bezeichnung: Milk Fat Globule EGF Factor 8 (MFGE8) (MFGE8 Produkte)

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Pathways: SARS-CoV-2 Protein Interaktom