MID1 ELISA Kit

Produktnummer ABIN2043975

Dieses Maus MID1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus MID1 zu quantifizieren.

Kurzübersicht für MID1 ELISA Kit (ABIN2043975)

Target: MID1 (Midline 1 (MID1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails MID1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Marke: BioAssay™

Sensitivität: < 0.052 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Midline 1 (MID1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midline 1 (MID1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Midline 1 (MID1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Midline 1 (MID1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Midline 1 (MID1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MID1

Target: MID1 (Midline 1 (MID1))