MTAP ELISA Kit

Produktnummer ABIN2043971

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus MTAP.

Kurzübersicht für MTAP ELISA Kit (ABIN2043971)

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails MTAP ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.053 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Methylthioadenosine Phosphorylase (MTAP) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylthioadenosine Phosphorylase (MTAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylthioadenosine Phosphorylase (MTAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylthioadenosine Phosphorylase (MTAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Methylthioadenosine Phosphorylase (MTAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process