MAT2A ELISA Kit

Produktnummer ABIN2043967

Ratte MAT2A ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte MAT2A.

Kurzübersicht für MAT2A ELISA Kit (ABIN2043967)

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails MAT2A ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.054 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Methionine Adenosyltransferase II Alpha (MAT2a) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionine Adenosyltransferase II Alpha (MAT2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Methionine Adenosyltransferase II Alpha (MAT2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MAT2A

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Andere Bezeichnung: Methionine Adenosyltransferase II alpha (MAT2a)

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process