MIA ELISA Kit

Produktnummer ABIN2043953

Produktdetails MIA ELISA Kit

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.25 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Melanoma Inhibitory Activity Protein 1 (MIA1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Inhibitory Activity Protein 1 (MIA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Inhibitory Activity Protein 1 (MIA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Inhibitory Activity Protein 1 (MIA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Melanoma Inhibitory Activity Protein 1 (MIA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Andere Bezeichnung: Melanoma Inhibitory Activity Protein 1 (MIA1) (MIA Produkte)

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