MGP ELISA Kit

Produktnummer ABIN2043939

Produktdetails MGP ELISA Kit

Target: MGP (Matrix Gla Protein (MGP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.37 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Matrix Gla Protein (MGP) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Gla Protein (MGP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Gla Protein (MGP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Gla Protein (MGP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Matrix Gla Protein (MGP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MGP

Target: MGP (Matrix Gla Protein (MGP))

Abstract: MGP Produkte

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