CCL15 ELISA Kit

Produktnummer ABIN2043905

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human CCL15.

Kurzübersicht für CCL15 ELISA Kit (ABIN2043905)

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62-1000 pg/mL

Applikation: ELISA

Produktdetails CCL15 ELISA Kit

Untere Nachweisgrenze: 15.62 pg/mL

Marke: BioAssay™

Sensitivität: < 5.3 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Macrophage Inflammatory Protein 5 (MIP5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 5 (MIP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 5 (MIP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 5 (MIP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Macrophage Inflammatory Protein 5 (MIP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CCL15

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Andere Bezeichnung: Macrophage Inflammatory Protein 5 (MIP5)