Ceramide Synthase 2 ELISA Kit

Produktnummer ABIN2043895

Human Ceramide Synthase 2 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Ceramide Synthase 2.

Kurzübersicht für Ceramide Synthase 2 ELISA Kit (ABIN2043895)

Target: Ceramide Synthase 2 (CERS2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails Ceramide Synthase 2 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.061 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Longevity Assurance Homolog 2 (LASS2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Longevity Assurance Homolog 2 (LASS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Longevity Assurance Homolog 2 (LASS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Longevity Assurance Homolog 2 (LASS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Longevity Assurance Homolog 2 (LASS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Ceramide Synthase 2

Target: Ceramide Synthase 2 (CERS2)

Andere Bezeichnung: Longevity Assurance Homolog 2 (LASS2)