Lipase A ELISA Kit

Produktnummer ABIN2043893

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Maus Lipase A.

Kurzübersicht für Lipase A ELISA Kit (ABIN2043893)

Target: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails Lipase A ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.117 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Lipase A, Lysosomal Acid (LIPA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase A, Lysosomal Acid (LIPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase A, Lysosomal Acid (LIPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase A, Lysosomal Acid (LIPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lipase A, Lysosomal Acid (LIPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu Lipase A

Target: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Andere Bezeichnung: Lipase A, Lysosomal Acid (LIPA)