LASP1 ELISA Kit

Produktnummer ABIN2043889

Human LASP1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human LASP1.

Kurzübersicht für LASP1 ELISA Kit (ABIN2043889)

Target: LASP1 (LIM and SH3 Protein 1 (LASP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails LASP1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.065 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: LIM And SH3 Protein 1 (LASP1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM And SH3 Protein 1 (LASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM And SH3 Protein 1 (LASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM And SH3 Protein 1 (LASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of LIM And SH3 Protein 1 (LASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu LASP1

Target: LASP1 (LIM and SH3 Protein 1 (LASP1))