LDHD ELISA Kit

Produktnummer ABIN2043869

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human LDHD.

Kurzübersicht für LDHD ELISA Kit (ABIN2043869)

Target: LDHD (Lactate Dehydrogenase D (LDHD))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails LDHD ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.59 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Lactate Dehydrogenase D (LDHD) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase D (LDHD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactate Dehydrogenase D (LDHD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactate Dehydrogenase D (LDHD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lactate Dehydrogenase D (LDHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu LDHD

Target: LDHD (Lactate Dehydrogenase D (LDHD))

Pathways: Ribonucleoside Biosynthetic Process