LCT ELISA Kit

Produktnummer ABIN2043867

Dieses Ratte LCT ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte LCT zu quantifizieren.

Kurzübersicht für LCT ELISA Kit (ABIN2043867)

Target: LCT (Lactase (LCT))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails LCT ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.116 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Lactase (LCT) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactase (LCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactase (LCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactase (LCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lactase (LCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu LCT

Target: LCT (Lactase (LCT))