KRT40 ELISA Kit

Produktnummer ABIN2043857

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human KRT40.

Kurzübersicht für KRT40 ELISA Kit (ABIN2043857)

Target: KRT40 (Keratin 40 (KRT40))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails KRT40 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.064 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Keratin 40 (KRT40) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 40 (KRT40). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 40 (KRT40). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 40 (KRT40), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 40 (KRT40) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu KRT40

Target: KRT40 (Keratin 40 (KRT40))